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CEEHRC / For Scientists / Protocols and Standards /

IHEC Guidelines

The International Human Epigenomics Consortium (IHEC) has established an Assay Standards and Quality Control Working Group (Chaired by CEEHRC Network Leader Martin Hirst) to define the assays required for three distinct classes of reference epigenome, and to define standardized protocols and quality control metrics for each assay. The Working Group's recommendations are intended to provide a framework for the definition of reference epigenomes to be included within the International Human Epigenome Consortium. These recommendations are minimal standards based on current knowledge of the elements contributing to epigenomic regulation in human cells and the current state of epigenomic mapping technologies, and are reviewed and updated annually.

Standard Operating Procedures

As an additional resource, the specific epigenomics protocols used at the BC Cancer's Genome Sciences Centre are listed below. The linked documents represent the latest versions of all SOPs as of May 2022; if you require a different version of any of our SOPs or past protocols, please contact us.

Wet Lab
Sample preparation, nucleic acid extraction and QC
Whole genome sequencing (WGS)
Whole genome bisulfite sequencing (WGBS)
Chromatin immunoprecipitation sequencing (ChIP-Seq)
Validation and QC of ChIP-Seq reagents